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Table legends: 
  • Cryoprotected conditions: Cryoprotected
  • Suitable for membrane proteins: Membrane protein
Initial screening (Sparse matrix)

 

Name of screen: Screen type: Supplier: Legend: Composition: Product number:
Classic I SuiteDefine crystallization conditions of soluble protein, pH(4.6-9.0)Qiagen pdf130701
Classic II SuiteDefine crystallization conditions of soluble protein, pH(3.5-8.5)Qiagen pdf130723
JCSG Core Suite IDefine crystallization conditions of a new protein based on the JCSGQiagen pdf130724
JCSG Core Suite IIDefine crystallization conditions of a new protein based on the JCSGQiagen pdf130725
JCSG Core Suite IIIDefine crystallization conditions of a new protein based on the JCSGQiagen pdf130726
JCSG Core Suite IVDefine crystallization conditions of a new protein based on the JCSGQiagen pdf130727
JCSG+ SuiteDefine crystallization conditions of a new protein based on the JCSGQiagen pdf130720
Pi-minimal_HTS screenBased on a novel combinations of precipitants, buffers and additives condition for the screening of soluble proteins. pH(4.0-9.5)Jena Bioscience pdfCS-211L
Morpheus_HT-96Simple and effective 3D grid design covering a range of pH, PEGs and salt additives. 49 low molecular weight ligands observed to promote initial crystal formation.Molecular DimensionsCryoprotectedpdfMD1-47
Morpheus-II_HT-96Morpheus II is a follow-up to the best-selling 96-condition screen, MorpheusMolecular Dimensions pdfMD1-92
Clear Strategy_HT-96 Screen IDefine crystallization conditions of soluble protein. Maintain ‘folding homogeneity’ of protein. pH(5.5 / 6.5 / 7.5 / 8.5)Molecular DimensionsCryoprotectedpdfMD1-31
Clear Strategy_HT-96 Screen IIDefine crystallization conditions of soluble protein. Maintain ‘folding homogeneity’ of protein. pH(5.5 / 6.5 / 7.5 / 8.5)Molecular DimensionsCryoprotectedpdfMD1-32
MemGold_HT-96Membrane protein screen based on the Protein Data Bank. PEGs and salt additives. Based on 130 membrane protein structures in the PDB. Covering a range of pH(5.2-8.5)Molecular DimensionsMembrane proteinpdfMD1-41
MemGold2_HT-96This screen targets all alpha helical types of Prokaryotic and Eukaryotic membrane proteinsMolecular DimensionsMembrane proteinpdf MD1-64
Screen Membrane HTS72 of the most successful buffers for crystallization of membrane proteinsJena BioscienceMembrane proteinpdfCS-305L
LMB Crystallization ScreenA 96 conditions sparse matrix screen based on optimised and published condtitions from the LMB-MRC, Cambridge UK.Molecular DimensionspdfMD1-99

 

Alternative screens (buffer and pH customized by the user)

(available on demand, contact the facility)

Name of screen: Screen type: Supplier: Legend: Composition: Product number:
Clear Strategy™ Screen I24 stock solutions testing a range of salts and PEGs. User should provides 4 x 1M buffers allowing full control of the pH.Molecular Dimensions pdf130704
Clear Strategy™ Screen II24 stock solutions testing a range of salts, organic solutions, cations, PEG 4000 together with calcium acetate. User should provides 4 x 1M buffers allowing full control of the pH.Molecular Dimensions pdf130716

 

Optimization screens (Grid screens - Single precipitant)
Name of screen: Screen type: Supplier: Legend: Composition: Product number:
PEGs SuiteTest a wide range of PEG molecular weights (200 up to 20000) and four different pH value. Test also the effect of PEG 3350 with a wide spectrum of low concentration saltsQiagen pdf130704
PEGs II SuiteUse polyethylene glycols of varying molecular weightsQiagen pdf130716
AmSO4 SuiteFor refinement of promising conditions containing AmSO4Qiagen pdf130705
PACT SuiteDifferent combinations of PEGs and ions to test effect of anions and cations and pHQiagen pdf130718
pHClear Suite IFor systematic analysis of precipitant concentration and pH(4-9)Qiagen pdf130709
pHClear Suite IIFor systematic analysis of precipitant concentration and pH(4-9)Qiagen pdf130705
Protein Complex SuiteOptimized for protein-protein complexQiagen pdf130715
Nucleix SuiteSuited for protein-nucleic acid complexQiagen pdf130719
Pentaerythritol_HTSBased on pentaerythritol polymers as precipitants. 4 polymers that differ from MPD and PEG's in size and nature. pH(4.6 / 6.5 / 7.5 / 8.5)Jena BioscienceCryoprotectedpdfCS-210L
MIDAS_HT-96Based on alternative polymeric precipitants. Salt and pH centred on physiological values. Ideal for protein and complexes. Designed to complement PEG and alt based-screens.Molecular DimensionsCryoprotectedpdfMD1-60
PGA LM_HT-96Based on poly-?-glutamic acid polymer as precipitant suitable for both globular and membrane protein crystallization. Non-denaturating conditions. pH(5 / 6.5 / 7.8)Molecular DimensionsMembrane proteinpdfMD1-51

 

 


LCP screens
(available on demand, contact the facility)
Name of screen: Screen type: Supplier: Legend: Composition: Product number:
Cubic ScreenThe LCP method works especially well when traditional methods have failed to yield crystals.Jena BioscienceMembrane proteinpdfCS-EB-LCP-B
 MemMeso HT-96 Designed to work in synergy with Lipidic Cubic PhaseMolecular DimensionsMembrane protein  pdfMD1-87

 

 

Additive screens

(available on demand, contact the facility)

Name of screen: Screen type: Supplier: Legend: Composition: Product number:
Additive_screen96 unique additives and their ability to influence the crystallizationHampton Research pdfHR2-428 
 MemAdvantage

An additive screen developed exclusively for membrane proteins. This screen targets all alpha helical types of Prokaryotic and Eukaryotic membrane proteins 

Molecular Dimensions  Membrane protein pdf MD1-70 

 


Customized user screens

The user can provide his own customized screen in a 96-well format

How do I decide which screens to use?

We recommend in general to start your crystallization trial with 3 or 4 sparse matrix as initial screening. The more you know about your protein, the better initial choice you can make. Then select few additional screens (sparse or grid screens) based on your preliminary results from your initial screening. You may need to consider to vary protein concentration if you have a massive precipitation quickly in most of the conditions.

How do I overcome screen redundacy?

Have a look at our tables (download pdf file here)

The C6 is a webtool which aims to simplify the crystallization landscape by showing the relationship between the different screens, and help you select an appropriate subset of the screens that we have available. C6 provides a list of all the currently available commercial screens and a breakdown on the individual contents of each well. You can use the C6 webtool also to find screens that contain a condition that you know your protein likes (or something very close). For example the literature may suggest that similar proteins have crystallised rapidly in ammonium sulfate and low MW PEGs, using the C6 Webtool you can search for all screens that contain conditions with these chemicals.